An Alternative Method for Cultivation of Lawsonia intracellularis

Method and System for Diagnosis of Lawsonia Intracellularis

The discovery of Lawsonia intracellularis

Lawson, G. H. K.; McOrist, S.; Sabri, J. and Mackie, R. A. (1993): Intracellular bacteria of porcine proliferative enteropathy: Cultivation and maintenance in vitro. J Clin Microbiol 31: 1136 – 1142. McOrist, S.; Jasni, S.; Mackie, R. A.; MacIntyre, N.; Neef, N. and Lawson, G. H. K. (1993): Reproduction of porcine proliferative enteropathy with pure cultures of ileal symbiont intracellularis. I...

Cultivation and characterization of Lawsonia intracellularis isolated from rabbit and pig.

Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. In this study, we improved the in vitro cultivation method of L. intracellularis to increase the passage efficiency and showed that L. intracellularis isolated from a rabbit and a pig have different antigenic properties. Bacteria should be recove...

How to Diagnose and Treat Lawsonia intracellularis

Proliferative enteropathy (PE) caused by Lawsonia intracellularis has emerged as a significant equine pathogen in recent years. Early diagnosis of this disease allows prompt initiation of appropriate therapy to help decrease the severity of this disease. Lawsonia intracellularis is an obligate intracellular, gram-negative, curve-shaped bacteria. In pigs, the organism results in lesions in the l...

1 TITLE : An alternative method for cultivation of

23 An alternative method for cultivation of Lawsonia intracellularis, an obligate intracellular 24 bacterium and causative agent of proliferative enteropathy, was described using Original Space 25 Bag® inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% 26 nitrogen. The flexibility of this protocol allows for the testing of various environmental 27 conditions for...